Dissertation Defense
Long-term Super Resolution on Cellular Dynamics Enabled by Phase Intensity Nanoscopy
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PASSCODE: GOBLUE
Emergent processes in living organisms and soft matter involve nanoscale reorganization, driving group-level movements and shape changes over time. However, photobleaching, caused by light-induced degradation of fluorophores, limits extended bioimaging in life sciences. To address this, two advanced imaging techniques, nonbleaching phase-intensity nanoscopy (PINE) and interferometric phase-intensity nanoscopy (iPINE), for long-term, high-resolution bioimaging. PINE achieves sub-10 nm resolution by separating phase and intensity using a multilayer thin film, enabling dynamic imaging of cellular reorganization over 250 hours without photobleaching. These nanoscopic changes correspond to larger scale movements, reflecting key interaction rules in biological self-organization. To minimize interference with biological functions, iPINE offers a label-free approach by combining interferometric scattering with phase-intensity modulation, allowing high sensitivity and resolution of adjacent nanometer-sized objects. Both techniques hold significant potential for advancing biology, biomedicine, and bioengineering by enabling precise, long-term observation of dynamic nanoscale processes.
CHAIR: Somin Lee