Imaging Engineering Tissue

Abstract:

A core challenge being addressed by the Imaging Thrust of the CELL MET Engineering Research Center is to answer the question: In engineered tissue platforms, how do we do volumetric imaging over wide fields of view with high resolution and at high speed? In this talk I will discuss the problem generically, beginning with three strategies for imaging volumetrically: widefield, confocal, and multiphoton microscopy. I will describe progress that we have made in particular for imaging modalities that are useful for engineered cardiac tissue. I will discuss the use of recently-engineered fluorescent voltage sensors to allow millisecond-scale temporal resolution in microscopy, and the advanced microscopes that we have been using to explore tissue structures. Finally, I will talk about cardiac micro tug (µTug) imaging and our progress in developing platforms for high-throughput screening, force measurement and control with optical feedback, and an all-optical non-contact tool for measuring  tissue stiffness.

Bio:

Dr. Thomas Bifano earned his Ph.D. in Mechanical Engineering from North Carolina State University and his M.S. and B.S. in Mechanical Engineering and Materials Science from Duke University. He has been a Professor at Boston University since 1988.

Dr. Bifano’s research focuses on modeling, design, production, and use of micro-electro-mechanical systems (MEMS) in optical applications. He is a founder and CTO of Boston Micromachines Corporation in Cambridge, MA, a leading producer of deformable mirrors for applications in astronomy, bio-imaging, and defense.

Zoom Link: https://bostonu.zoom.us/j/93315594357?pwd=eE4wQS9lZjA2aloyMkQ2dG5CS21kZz09

 Meeting ID: 933 1559 4357,  Passcode: 689715